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duox2  (Cusabio)


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    Cusabio duox2
    Duox2, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/duox2+polyclonal+antibody/pmc12067898-93-11-17?v=Cusabio
    Average 93 stars, based on 2 article reviews
    duox2 - by Bioz Stars, 2026-07
    93/100 stars

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    Fig. 2. Expression of Nox-Duox in human villous cytotrophoblast using RT-PCR and Sanger sequencing. (A) PCR products of cDNA from term placenta VCT (100 ng/well). Primers used targeted Nox1, Nox2, Nox3, Nox4, Nox5, <t>Duox1</t> or <t>Duox2.</t> Revelation is performed using UV light and Minibis transilluminator. (B) Sanger sequences obtained by Eurofins from PCR amplification products.
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    Image Search Results


    Fig. 2. Expression of Nox-Duox in human villous cytotrophoblast using RT-PCR and Sanger sequencing. (A) PCR products of cDNA from term placenta VCT (100 ng/well). Primers used targeted Nox1, Nox2, Nox3, Nox4, Nox5, Duox1 or Duox2. Revelation is performed using UV light and Minibis transilluminator. (B) Sanger sequences obtained by Eurofins from PCR amplification products.

    Journal: Archives of biochemistry and biophysics

    Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

    doi: 10.1016/j.abb.2023.109787

    Figure Lengend Snippet: Fig. 2. Expression of Nox-Duox in human villous cytotrophoblast using RT-PCR and Sanger sequencing. (A) PCR products of cDNA from term placenta VCT (100 ng/well). Primers used targeted Nox1, Nox2, Nox3, Nox4, Nox5, Duox1 or Duox2. Revelation is performed using UV light and Minibis transilluminator. (B) Sanger sequences obtained by Eurofins from PCR amplification products.

    Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

    Fig. 4. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5, anti-DUOX1 and anti-DUOX2 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 50 μm. On the right of the panel, magnification of term chorionic villi was done. Scale bar = 20 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis.

    Journal: Archives of biochemistry and biophysics

    Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

    doi: 10.1016/j.abb.2023.109787

    Figure Lengend Snippet: Fig. 4. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5, anti-DUOX1 and anti-DUOX2 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 50 μm. On the right of the panel, magnification of term chorionic villi was done. Scale bar = 20 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis.

    Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

    Techniques: Immunostaining, Negative Control

    Fig. 5. Expression of NOX family proteins during Pre-eclampsia (PE). Immunoblot using (A) NOX1-3-5 (B) NOX4 and DUOX1 antibodies is performed on physiological term placentas (control, n = 10) and pre-eclamptic placentas (EARLY-PE < 34GW, n = 11 and LATE-PE > 34 GW, n = 7). Vinculin is used as loading control. Graphs represent the total amount of each protein relative to Vinculin level determined by quantification of immunoblot using ImageStudiolight from LICOR. The mean is reflected by long horizontal lines and standard deviations by short horizontal lines. Statistical analysis was performed using Mann-Whitney test. *p < 0,05.

    Journal: Archives of biochemistry and biophysics

    Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

    doi: 10.1016/j.abb.2023.109787

    Figure Lengend Snippet: Fig. 5. Expression of NOX family proteins during Pre-eclampsia (PE). Immunoblot using (A) NOX1-3-5 (B) NOX4 and DUOX1 antibodies is performed on physiological term placentas (control, n = 10) and pre-eclamptic placentas (EARLY-PE < 34GW, n = 11 and LATE-PE > 34 GW, n = 7). Vinculin is used as loading control. Graphs represent the total amount of each protein relative to Vinculin level determined by quantification of immunoblot using ImageStudiolight from LICOR. The mean is reflected by long horizontal lines and standard deviations by short horizontal lines. Statistical analysis was performed using Mann-Whitney test. *p < 0,05.

    Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

    Techniques: Expressing, Western Blot, Control, MANN-WHITNEY

    Fig. 6. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5 and anti-DUOX1 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 20 μm. On the right of the panel, magnification of term chorionic villi is done. Scale bar = 10 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis

    Journal: Archives of biochemistry and biophysics

    Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

    doi: 10.1016/j.abb.2023.109787

    Figure Lengend Snippet: Fig. 6. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5 and anti-DUOX1 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 20 μm. On the right of the panel, magnification of term chorionic villi is done. Scale bar = 10 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis

    Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

    Techniques: Immunostaining, Negative Control